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AP-1 is a protein dimer composed of a heterodimer of Fos and Jun proteins

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For the neuronal culture, hippocampi were dissected in HBSS, and digested for 1 h at 37 °C in DMEM supplemented with 25 U/ml papain (Worthington Biomedical Corp., USA), 0.2 mg/ml cysteine (Merck, Germany), 1 mM CaCl 2 , and 0.5 mM EDTA